CyTOF 2.1 is an upgraded version of the second generation mass cytometer, capable of measuring 135 different mass channels (75-209 amu) at the peak throughput of around 1000 cells/sec. It analyzes individual cells labeled with stable metal isotopes using state-of-the-art inductively coupled plasma time-of-flight technology and has the capability to simultaneously resolve multiple elemental probes per cell at high acquisition rates, thereby maximizing the per-cell information obtained from a single sample.
Mass cytometry employs elemental tags that are uncommon in biological systems. Cells stained with metal-conjugated probes in a single-cell suspension are introduced into the CyTOF 2 and undergo a multistep process within the instrument to detect these metal tags and record the identity and amount of each probe on each cell.
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