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Cytometry Facility

Guidelines for Registration

Staff members of the Cytometry Facility show you how to operate the instruments with your samples. Additionally, preparation techniques and methods are communicated to minimize sample - fluidic interactions. 

Users that gained sufficient knowledge in flow cytometer handling are allowed to operate the instruments independently. The facility staff members instruct every user personally and decide for each user individually whether the education is appropriate. This guarantees safety for the user, the sample and the flow cytometer. Guarantee on device performance and / or experiment outcome especially cell sorts however is not given. Booked instrument time will be charged. The Cytometry Facility reserves the right to teach new operators on user samples. Fees are published on the facility website and follow the rules of SNF, EU and UZH. 

Cancellation policy:

  • If you cannot attend an appointment with our staff (sorting service or support and power user trainings), cancelation is possible up to 24 hours prior to start of the meeting. No-show situations without notification need to be be fully charged. For cancelling on short notice (less than 24h) we will charge 1 hour of the respective service.
  • All independent users of analyzers and sorters can cancel/modify bookings up to 24h in advance using the respective implementation within instrument booking calendar. 

All work published that include results obtained using resources of the Cytometry Facility should be acknowledged and a copy of the publication should be sent to Claudia Dumrese. Examples for acknowledgments
...Flow cytometry was performed with equipment of the Cytometry Facility, University of Zurich...
...Flow cytometry was performed with support of the Cytometry Facility, University of Zurich...
...The authors acknowledge the assistance and support of the Cytometry Facility, University of Zurich for performing cell sorting experiments...

The user and their principle investigators subscribe with the registration to treat material and instruments carefully, report any irregularities to the facility staff, book via the official facility instrument booking system before using a cytometer and accept the facility guidelines including cancellation policy.

Collaboration

Scientific projects can be performed in collaboration with the Cytometry Facility on request. Collaborations are characterized by intense scientific input, extensive preparation and analysis of the samples but also unusual operation of instruments. Whether a collaboration is established between scientific core facility staff and a research group is decided by the Director of the Cytometry Facility after a project discussion. Staff members involved in collaboration must be authors on the resulting publication. 

Service

Staff members of the Cytometry Facility will do all the preparative work on a service like basis. The Cytometry Facility will charge the staff time used for all preparative steps, experiment planning, the instruments usage and operator time for running the instrument and/or help on data analysis according to SNF recommendations. EU rules and UZH Weisung.

Consultation

Methodical problems in acquisition of cytometry samples or data analysis can be discussed with the staff of the Cytometry Facility and is in general free of charge. 

Fees

Instruments are charged automatically based on the entries in the reservation system or computer login time. Booked and instrument usage time is used for billing. Accounting will be done every three months (1st of March, 1st of June, 1st of September, 1st of December). Changes of the instrument fees will be communicated individually by email to every PI beforehand. Fees are published on the homepage

Reservation and use of systems maintained by the Cytometry Facility

Access to all systems is possible during legal working hours for users having the necessary knowledge to work independently and safe. Reservation of systems is mandatory prior that work is started. Reservations must be done for the whole time the system is used. Reservation of systems without usage is not allowed and will be billed. Logging to the systems must be done with the personal username and password. Logging using a different username and password is not allowed. Lost login information or password reset can always be obtained from the IT-Infrastructure website of the Cytometry Facility. Booking regulations for all instruments are communicated in the facility booking calendar and need to be fulfilled by the users. Problems with systems should be communicated via instrument feedback form.  We communicate system repairs immediately to all users having booked the device in order to enable them to book an alternative device. Repair status is constantly updated in the booking calendar of the respective device.

Biosafety

All work involving biosafety class 2 organisms on all our systems must be performed according to GLP and biosafety regulations of the Cytometry Facility, University of Zurich and the BAG (Bundesamt für Gesundheit). "Biosafty class" & "Material and Amount" are mandatory to be communicated truly via the corresponding fields in the instrument booking calendar.

PI and Biosafety officers of the user are responsible to ensure that the users fulfill all biosafety rules in the rooms of the Cytometry facility, Lengg Research Building, Schlieren and USZ. The class 2 cell culture lab may be used for culturing and preparing cells for experiments performed with all systems at the Cytometry Facility.

Privacy

The manufacturer of the cytometers offer remote support. Please be aware that there is a possibility that the manufacturer might become aware of your name, should remote support be requested during operation. To prevent disclosing personal data afterwards, please always delete your name, user entry, user data after using the devices of the cytometry facility.