LSR II Fortessa with HTS - Schlieren

High-end BD SORP flow cytometer with 4 lasers and 18 measureable parameters (2 light-scatter signals and 16 fluorescent signals).

Available laser lines: 405 nm (violet), 488 nm (blue), 561 nm (yellow/green) and 640 nm (red). The filter setup allows detection of most standard fluorochromes (see optical configuration for details).

The cytometer can handle analysis rates of up to 40,000 events/sec and is equipped with an High Throughput Sampler (HTS) for different well type plates.

Important: Please filter all your samples prior to measurement ( ⇒ Falcon #352235) and perform all washing steps described in the SOP to avoid clogging of the system.

Samples can only be loaded in 5 ml FACS tubes made of polystyrene (clear plastic e.g. FAL352058), polypropylene tubes (milky plastic like e.g. falcons or Eppis) will not pressurize. Minimum sample volume is 200 ul.
If you have sample volumes < 200 ul you can place an additional micronic tube in the 5 ml tube (e.g. Vitaris, Product no.: 32022-MIC).

 

The instrument is located in Schlieren, Wagistrasse 12, in room WAD-K-104.
Telephone: 044 63 53 490

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Biosafety note: The Fortessa in Schlieren runs on a biosafety 2 permit. Please treat the workspace accordingly and indicate the biosafety level of your experiment in the instrument booking system.

If there is any doubt about the biosafety classification of your experiments please contact us  info@cytometry.uzh.ch

 

Usage of the instrument requires an introduction that is provided by the facility.

New users ►  please register with the facility.

Registered users ► request an introduction by email to  info@cytometry.uzh.ch