FACSAria III 5L - Irchel - 1

Sorter Specifications

5 lasers, 405 nm (violet), 445 nm, 488nm (blue), 561 nm (yellow/green) and 633 nm (red) are spatially separated for excitation of 16 parameters as described in the optical layout.

70 µm, 85 µm, 100 µm and 130 µm nozzles are available for cell sorting of material ranging from bacteria to fat cells or tubules.

Please consult our guidelines for sample preparation if you are unclear about how to prepare your cells for the sorting.

Sorted cells can be collected in up to

  • 4 x 5 ml tubes,
  • 2 x 15 ml falcons,
  • 4 x 1.5 ml eppis,
  • 6 well, 24 well, 48 well, 96 well and 384 well plates
  • onto microscopic slides or custom made collection devices

We additionally offer custom made sample holders with following specifications:

  • 4 x 2 ml eppis
  • 1 x 5 ml tube and 3 x 1.5/2 ml eppis
  • 1 x 15 ml falcon and 3 x 5 ml tubes
  • 1 x 50 ml falcon and 2 x 5 ml tubes

Samples as well as collected cells can be kept at desired temperature (4° - 42°C).

An aerosol management system allows cell sorting up to biosafety level 2.

The sorter is operated by independent power users, power user in training and via operator based sorts.

 

Operator based sorts & training are available on request Mo - Fr from 09:00 - 17:00.

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Contact info@cytometry.uzh.ch for further questions.

Telephone next to the sorter:  044 63 55 353