The NanoAnalyzer is a dedicated flow cytometer built specifically for analyzing nano-sized particles. Based on light scattering and fluorescence it can detect small biological particles down to ~40 nm in size.
The machine is equipped with a 488nm and 640 nm laser and records SSC and up to two fluorescent channels with highly sensitive photon avalanche diodes.
|Optimal sample concentration||1 x 108 - 1 x 109 particles/ml|
|Sample flow rate||5 - 60 nl/min|
|Sheath flow rate||10 - 40 µl/min|
|Calibrated sample size range for SSC||
S16M-Exo: 40 - 200 nm
S17M-MV: 150 - 1000 nm
|Maximum particle size to be loaded||1 µm|
|Sample loading||0.6 ml microtubes|
|Optimal sample event rate||2000 - 12000 events/min|
Note: The samples can be exclusively loaded in 0.6 ml microtubes (MCT-060-L-C, Axygen or alternative supplier) which are not provided by the facility!
Compensation cannot be applied in the Nanoanalyzer software but data can be exported as FCS files to calculate compensation in different software.